ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. III. THE INCORPORATION OF PYRIMIDINE AND PURINE ANALOGUES INTO DEOXYRIBONUCLEIC ACID

Enzymatic Synthesis of Deoxyribonucleic Acid. Iii. the Incorporation of Pyrimidine and Purine Analogues into Deoxyribonucleic Acid.

The role of deoxyribonucleic acid in ribonucleic acid synthesis. IV. The incorporation of pyrimidine and purine analogues into ribonucleic acid.

The synthesis of ribonucleic acid from ribonucleoside triphosphates by an enzyme system dependent on deoxyribonucleic acid has been described (2-7). The base composition of the RNA produced is identical with that of the DNA which is added. This result can be attributed, by an analogy with the complementary base model of DNA structure proposed by Watson and Crick (8), to hydrogen bonding by succ...

Enzymatic Synthesis of Deoxyribonucleic Acid. Deoxyribonucleic Acid By

MISSOURI The enzymatic synthesis of DNA1 in sufficient excess over the DNA used to prime the reaction21 a has enabled us to examine the chemical composition of the synthetic product. As has already been demonstrated, this DNA consists of deoxy-nucleotides Linked by typical 3'-5' phosphodiester bonds, and all four of the deoxy

Enzymatic synthesis of deoxyribonucleic acid.

Determination of deoxyribonucleotide sequence in a deoxyribonucleic acid molecule is important from both the chemical and genetic points of view. It is also essential for answering the question of whether DNA synthesized in vitro by polymerase (1, 2) is a faithful copy of the nucleotidel sequence of the primer DNA. Although enzymatically synthesized DNA has the same over-all nucleotide composit...

The role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin.

* Report of work supported by the U.S. Public Health Service, grants no. RG-3977, RG-5143, and A-3102. We wish to acknowledge the fine technical assistance of Hedi Imfeld and the counsel and cooperation of F. William Studier. 1 Huang, R. C., N. Maheshwari, and J. Bonner, Biochem. Biophys. Res. Comm., 3, 689 (1960). 2 Bonner, J., R. C. Huang, and N. Maheshwari, these PROCEEDINGS, 47, 1548 (1961)...